What is it used for?
Real-time detection and monitoring of biomolecular binding events
- Specificity – How specific is the binding between two molecules
- Concentration – How much of a given molecule is present or active
- Kinetics – What is the rate of association / dissociation
- Affinity – How strong is the binding?
Interactions between proteins, nucleic acids, lipids, carbohydrates and even whole cells can be studied (molecules as small as 100 Da).
How does it work?
- Quantitative measurements are dependent on the immobilisation of a target molecule to the surface of a sensor chip.
- The analyte is delivered to the chip surface using integrated micro fluidic cartridges
- Surface Plasmon Resonance detects changes in the mass in the aqueous layer close to the sensor chip surface by measuring changes in the refractive index
Examples of how the WISB community are using this equipment
- Determination of binding constants for a selection of DNA-binding proteins (PI Christophe Corre)
- Determining the affinity of protein-protein and protein-ligand interactions (PI Greg Challis)
- How small peptides/molecules disturb protein-protein interactions (PI John McCarthy)
Access to the equipment
Please contact Sarah Bennett (firstname.lastname@example.org) for access to the equipment. We welcome both internal and external enquires.