We have had another successful year at the 2021 IGEM Giant Jamboree. The Warwick Team, CREscent, were awarded a Gold medal and you can watch their promotional video here.
More about the team…
Warwick’s 2021 team, CREscent, attempted to design a rapid detection method for a type of bacteria resistant to carbapenems, a last-resort antibiotic. These bacteria are often found in hospitals, and infections are extremely difficult to treat so isolation of infected patients is one of the only ways to contain its spread. Current detection methods for Carbapenem Resistant Enterobacteriaceae (CRE) are very slow and can take up to 4 days to return a positive result, exposing hospitals to potential unchecked spread of CRE in the meantime.
Therefore, we designed rapid detection method for hospitals, by targeting a carbapenem resistance gene in E. coli using CRISPR. If the RNA molecule produced by this gene is detected, a different RNA is transcribed, which causes fluorescence. This acts as a visual identifier. The idea was that a swab would be taken in a hospital and placed into a test tube. If the bacteria present are resistant to carbapenems then the tube will glow green under UV light.
Read more about their project on their wiki: