Andor/Nikon Spinning Disk Confocal Laser Microscope

What is it used for?

Live cell imaging with reasonable contrasts and minimal photobleaching.

Key Features

  • Lasers – 405, 488, 561 and 640 nm
  • Filters for fluorescent proteins – CFP/EGFP/YFP/mCherry and DAPI
  • Objectives – 100x 1.45NA, 60x 1.4NA, 40x 1.0NA. Auxiliary magnification at 1.5x
  • 2 x EMCCD 1k x 1k pixel camera, providing 65nm per pixel sampling with the 100x objective
  • Perfect focus system
  • Motorised stage
  • FRAP/PA unit
  • Environmental chamber

Examples of how the WISB community are using the Spinning disk microscope?

  • Examining the ageing process in yeast (PI McCarthy)
  • Confocal and live microscopy experiments using human mammalian cells and observing differences in them upon infection and phage addition (PI Sagona)

Andor/Nikon Widefield Fluorescence Microscopes (x2)

What are they used for?

Imaging and time lapse microscopy

Key Features

  • Filters for fluorescent proteins – CFP/EGFP/YFP/mCherry and DAPI
  • Objectives – 100x 1.45NA, 60x 1.4NA, 40x 1.0NA and 10x 0.45NA. Auxiliary magnification at 1.5x
  • One system with 0.5k x 0.5k EMCCD camera system
  • One system with 2k x 2k sCMOS camera system
  • Perfect focus system
  • Motorised stage
  • Environmental chamber. sCMOS system has CO2 control
  • State of the art LED illumination

Examples of how the WISB community are using the widefield microscopes?

  • Determine the ability of marine bacterial strains to degrade different kind of plastics (PI Christie-Oleza)
  • Imaging yeast to understand the relationship between translation machinery/pathway and ageing process (PI McCarthy)

WISB has been funded by the above bodies

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