Agilent 1260 HPLC

What is it used for?

Quantitative analysis and preparative separations of components in a chemical mixture.

Key Features

  • 1260 Infinity Quaternary pump. Maximum pressure 600 bar, flow rate from 0.2ml/min up to 10ml/min with inline degasser
  • Dual loop autosampler
  • Diode array detector (190 to 950 nm) with built in holmium oxide filter
  • Standard and high flow cells
  • Temperature controlled fraction collector
  • Thermostatted column compartment

Examples of how the WISB community are using the HPLC

  • Chromatographic separation of major metabolites released by microbes in defined culture media (PI Soyer)
  • Purification of natural products from Synechococcus sp. in axenic cultures and artificial, metabolically linked communities (PI Corre)

Bruker maXis II UHR-TOF LC-MS

What is it used for?

The identification and structural elucidation of novel and known natural products and biosynthetic intermediates in microbial culture extracts.

Protein mass spectrometry to characterise biosynthetic enzymes and intermediates that are covalently-attached to them.

Key features

  • Electrospray ionisation quadrupole Time-of-Flight MS for ultra-high-resolution exact mass and true isotopic measurements in both MS and MS/MS modes
  • Full sensitivity resolution of >80000
  • Mass range 20-40,000 m/z
  • Dionex UHPLC
  • CaptiveSpray nanoBooster – revolutionary spray source providing unmatched sensitivity, resists plugging and provides reproducible uninterrupted flow
  • Atmospheric pressure chemical ionisation II Ion source for LC Coupling

Examples of how the WISB community are using the LC-MS

Analysis of metabolic products of natural and engineered biosynthetic pathways; characterisation of biosynthetic intermediates covalently-attached to polyketide synthase and nonribosomal peptide synthetase multi-enzymes (PI Challis)

ThermoFisher Scientific ICS-5000 Dual Gradient RFIC Ion Chromatograph

What is it used for?

Quantitative analysis of cations, anions, organic acids, amino acids and carbohydrates in a mixture.

Key features

  • Dual channel system enables simultaneous analysis of anions and cations from the same sample injection
  • Eluent Generation system
  • Thermal Compartment for precise temperature control from 5 to 85 ºC
  • Conductivity Detector
  • Electrochemical Detector
  • Autosampler

Example of how the WISB community are using the IC

Chromatographic separation of major metabolites released by microbes in defined culture media (PI Soyer)

WISB has been funded by the above bodies

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