Becton Dickinson Fortessa Flow Cytometer

What is it used for?

A laser based cell analysis technique used for cell counting, biomarker detection and protein engineering.

Key Features

  • Laser setup –
    • 355nm UV (20 mW) / 2 fluorescence detectors
    • 405nm Violet (50 mW) / 6 fluorescence detectors
    • 488nm Blue (50 mW) / 2 fluorescence detectors
    • 561nm Yellow Green (50mW) / 5 fluorescence detectors
    • 640nm Red (40 mW) / 3 fluorescence detectors
  • Low, medium and high acquisition controls with fine tuning option
  • Optional high throughput sampler for cell analysis from a 96 well plate

Example of how the WISB community are using the Fortessa

  • Understand the relationship between translation machinery/pathway and ageing process in budding yeast (PI McCarthy)
  • Analysis of self-sustained synthetic microbial communities (i.e. phototrophic-heterotrophic interactions) (PI Christie-Oleza)

Becton Dickinson FACSAria Fusion Cell Sorter

What is it used for?

Sorting a heterogeneous mixture of cells into separate containers based upon specific light scattering and fluorescent characteristics.

Key Features

  • Same laser and detector arrangement as the Fortessa
  • Ability to sort into tubes (2 or 4 way), plates (96 and 384 well), petri dishes and slides.
  • Enclosed in a Class 2 biosafety cabinet providing operator and sample protection, with an aerosol management system.
  • 4 nozzle sizes: 70μm, 85μm, 100μm and 130μm
  • Temperature control system for maintaining sorted cells at required temperature
  • Stability over long sorts with ‘sweet spot’ technology

Example of how the WISB community are using the Fusion cell sorter

  • Single cell sorting to analyse the technical and biological sources of noise in animal cells – in cell culture (PI Hebenstreit)
  • Bulk cell sorting human and murine cell lines to study microtubule organisation, microtubule motors and microtubule associated proteins (PI Straube)

WISB has been funded by the above bodies