Tecan Freedom EVO 200

What is it used for?

Automated high throughput techniques, such as screening, plate replication, DNA extractions, transformations, colony picking, culture inoculation – growth – analysis.

Key features

We have two robotic platforms, each equipped with an 8-channel liquid handling arm, a 96 multichannel arm and a robotic manipulator arm.  The two systems have storage carousels and holders for reagents and multi-well plates.

Robot 1 is equipped with a shaking plate, heating and cooling plates, a vacuum filtration system and a thermocycler.  Robot 2 in equipped with another shaking plate, colony picker, incubators and a plate reader.  There is a shuttle between the two Robots for plate transfer.

The two systems can be programmed to carry out repetitive, high throughput tasks, without the need for human interaction.

Example applications

• Antimicrobial MIC hit detection and synergy screening (collaboration with John Moat, Director of the Antimicrobial Screening Facility)
• Combined plate replication and yeast transformation (PI Ntoukakis and Schafer)
• Combinatorial assembly approach for the construction and assay of recombinant proteins in yeast (PI John McCarthy)